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Year : 2016  |  Volume : 34  |  Issue : 4  |  Page : 526-528

Improved detection of Shigella using Escherichia coli medium enrichment: Polymerase chain reaction from stool samples


1 Department of Microbiology, Dr. RPGMC, Kangra, Himachal Pradesh, India
2 Department of Microbiology, SDM Medical College, Dharwad, Karnataka, India
3 Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, India

Correspondence Address:
N Taneja
Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.195355

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Laboratory diagnosis of shigellosis using conventional culture technique is limited by lower sensitivity and higher turnaround time. Here, we have evaluated the role of polymerase chain reaction from stool samples after enrichment in Escherichia coli medium for detection of Shigellae. The technique not only increased the sensitivity but also decreased the turnaround time.






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2004 - Indian Journal of Medical Microbiology
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