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 ORIGINAL ARTICLE
Year : 2006  |  Volume : 24  |  Issue : 1  |  Page : 25-29

Detection of biofilm formation among the clinical isolates of staphylococci: An evaluation of three different screening methods


1 New Drug Discovery Research, Ranbaxy Research Laboratories, Gurgaon-122 001, Haryana, India
2 Department of Biosciences, Jamia Millia Islamia University, Jamia Nagar, New Delhi-110 025, India

Correspondence Address:
T Mathur
New Drug Discovery Research, Ranbaxy Research Laboratories, Gurgaon-122 001, Haryana
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0255-0857.19890

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Purpose: The purpose of this study was to evaluate three methods for detection of biofilm formation in staphylococci. Methods: For detection of biofilm formation, 152 clinical isolates of Staphylococcus spp. were screened by tissue culture plate (TCP), Tube method (TM) and Congo red agar (CRA) method. Results: Of the 152 Staphylococcus spp. 88(57.8%) displayed a biofilm-positive phenotype under the optimized conditions in the TCP method and strains were further classified as high 22 (14.47 %) and moderate 60 (39.4 %) while in 70 (46.0 %) isolates weak or no biofilm was detected. Though TM correlated well with the TCP test for 18 (11.8 %) strongly biofilm producing strains, weak producers were difficult to discriminate from biofilm negative isolates. Screening on CRA does not correlate well with either of the two methods for detecting biofilm formation in staphylococci. Conclusion: The TCP method was found to be most sensitive, accurate and reproducible screening method for detection of biofilm formation by staphylococci and has the advantage of being a quantitative model to study the adherence of staphylococci on biomedical devices.






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2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04